alternative to wedge sequencing gels

xiaoming yi z3c20 at
Mon Apr 15 01:24:54 EST 1996

What is this based on? Refference?

In article <4kgd7n$ek6 at>, mbsmf says...
>After pre-running the gel, just before loading the samples, I routinely add 
>volume of 3M ammonium acetate to the lower buffer reservoir, which also 
>the TBE by 1/3. Then run the gel as usual. This closes up the band spacing in
>the bottom 1/3 of the gel.

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