DNA extn from high phenolic tissue?
s_mka1 at eduserv.its.unimelb.EDU.AU
Mon Apr 15 01:03:00 EST 1996
Does anyone have any good protocols/ideas for isolating relatively pure
DNA from plant tissue very high in phenolics? In particular, I'm trying
to get clean DNA from eucalyptus tissue. Currently I'm using 1%PVPP in
the extraction buffer, and will also try the soluble form, PVP.
However, DNA extracted so far is still quite resistant to digestion
and brown in colour! Any info would be greatly appreciated.
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