!!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!!
student at biocell.fundp.ac.be
Tue Apr 16 04:39:52 EST 1996
We need some help !
I'm trying to obtain the M-CSF promoter by PCR from total human
genomic DNA. DNA extraction method does'nt inhibit PCR amplification. We
have studied several parameters (T°, primer composition, genomic DNA
concentration) and conditions (buffers, DMSO). We have also tried a nested
PCR, without any result ! Is there anybody who can provide us some advices
? Thank in advance
Laboratory of Cellular Biochemistry,
Facultes Universitaires ND de la Paix,
61, rue de Bruxelles,
B-5000 Namur (Belgium).
Email: michet at biocell.fundp.ac.be
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