!!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!!

[MINET Emmanuel]

            We need some help !      

         I'm trying to obtain the M-CSF promoter by PCR from total human
genomic DNA. DNA extraction method does'nt inhibit PCR amplification. We
have studied several parameters (T°, primer composition, genomic DNA
concentration) and conditions (buffers, DMSO). We have also tried a nested
PCR, without any result ! Is there anybody who can provide us some advices
? Thank in advance

-- 
Michael HERMAN
Laboratory of Cellular Biochemistry,
Facultes Universitaires ND de la Paix,
61, rue de Bruxelles,
B-5000 Namur (Belgium).
Fax: ++/32/81/72.41.35.
Email: michet at biocell.fundp.ac.be