alternative to wedge sequencing gels

xiaoming yi z3c20 at ttacs1.ttu.edu
Mon Apr 15 23:48:22 EST 1996


can you tell me the refference?  I never tried this.

In article <4kgd7n$ek6 at mserv1.dl.ac.uk>, mbsmf says...
>
>After pre-running the gel, just before loading the samples, I routinely add 
1/2
>volume of 3M ammonium acetate to the lower buffer reservoir, which also 
dilutes
>the TBE by 1/3. Then run the gel as usual. This closes up the band spacing in
>the bottom 1/3 of the gel.
>
>Stuart
>




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