Help with Electroporation!!!
pemanuel at umabnet.ab.edu
Tue Apr 16 13:21:06 EST 1996
> >Why isn't my electroporation working?
> Someone has already indicted your volume of cells is high (300 uL
The 0.1 and 0.2 cm cuvettes are both excellent
> for prokaryotic cells because they achieve the high field strength
> needed to get the DNA into the smaller bacterial target.
Thanks. Problem solved. Seems to have been carry over salt from ligation.
Peter Emanuel Ph.D.
University of Maryland
School of Medicine
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