Help with Electroporation!!!

Peter pemanuel at
Tue Apr 16 13:21:06 EST 1996

> >Why isn't my electroporation working?
> >
> >Peter
> Someone has already indicted your volume of cells is high (300 uL 
 The 0.1 and 0.2 cm cuvettes are both excellent
> for prokaryotic cells because they achieve the high field strength 
> needed to get the DNA into the smaller bacterial target.
> M.

Thanks.  Problem solved.  Seems to have been carry over salt from ligation.

Peter Emanuel Ph.D.
University of Maryland
School of Medicine
410-706-429410-706-8012 FAX

More information about the Methods mailing list