help with ligation

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Wed Apr 17 05:53:27 EST 1996


I hate CIP!  It does damage ends, and takes ardous deactivating.  I use
SAP
(from USB, now Amersham I expect) and find it much friendlier.  Running
your
ligations out on a minigel is good advice, but I prefer 1:1 molar ratios
to
insert biased ones (call me a purist).

Anthony.



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