help with ligation

Richard Hastings mbxrh at mbn1.biochem.nottingham.ac.uk
Wed Apr 17 08:56:30 EST 1996


Doesn't T4 work best at 15C?
If i'm not in a hurry i ligate overnight too (I know most of the 
ligating occurs in the first hour or two).
Hope it helps,

Rich.
-- 

Richard Hastings
Department of Biochemistry
Queens Medical Centre
University of Nottingham
Nottingham
NG7 2UH
ENGLAND
0115 9249924  Ext.44789
Fax. 0115 9422225



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