inosine in oligonucleotides

Richard Hastings mbxrh at mbn1.biochem.nottingham.ac.uk
Wed Apr 17 08:47:42 EST 1996


Bob,

using the extinction co-efficient for individual nucleotides is very 
precise of you.  In our lab we use assume that on average, for an 
oligonucleotide, a single OD260 unit is equivalent to a concentration of 
37ug/ml.  We then convert that to uM (pmoles/ul) concentration using the 
molecular weight of the primer.
Don't know if that helps you at all, but good luck anyway.

Rich.

-- 

Richard Hastings
Department of Biochemistry
Queens Medical Centre
University of Nottingham
Nottingham
NG7 2UH
ENGLAND
0115 9249924  Ext.44789
Fax. 0115 9422225



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