inosine in oligonucleotides
mbxrh at mbn1.biochem.nottingham.ac.uk
Wed Apr 17 08:47:42 EST 1996
using the extinction co-efficient for individual nucleotides is very
precise of you. In our lab we use assume that on average, for an
oligonucleotide, a single OD260 unit is equivalent to a concentration of
37ug/ml. We then convert that to uM (pmoles/ul) concentration using the
molecular weight of the primer.
Don't know if that helps you at all, but good luck anyway.
Department of Biochemistry
Queens Medical Centre
University of Nottingham
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