Reply to RNA ISOLATION FROM WHOLE ORGANISM

Gerardo Medrano gerardo at N-JCENTER.COM
Wed Apr 17 08:02:09 EST 1996


> RNA isolation from whole organism rather than tissue or cells
> 
> john yon (yonjo00 at dons.ac.usfca.edu)
> Mon, 15 Apr 1996 21:33:49 +0100
> 
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> To: methods at net.bio.net
> 
> From: john yon <yonjo00 at dons.ac.usfca.edu>
> 
> Subject: RNA isolation from whole organism rather than tissue or cells
> 
> Date: Mon, 15 Apr 1996 21:33:49 +0100
> 
> Has anyone isolated RNA from a whole organism rather than just tissues
> 
> and cells? We're trying to isolate total RNA from whole earthworms
> which are homogenized. So far we have used the GTC homogenization
> buffer, where homogenates were loaded on to a cesium chloride cushion,
> 
> ultracentrifuged, and the RNA pellets. Unfortunately, we have been
> unsuccessful. We have taken all the necessary precautions to avoid
> degradation. Does anyone have any tips?
> 
> Thanks,
> 
> John Y. and Ric T.
> ----------------------------------------------------------------------
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>      question!"We have been isolating total RNA from whole mosquitoes for viral RNA PCR 
using TriZol Reagent. So far we have not had major problems. It is sold 
by Life Technologies, 1-800-828/6686.

Usual disclaimers.


Gerardo Medrano

East Flagler MCD
Palm Coast, Florida



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