Are plasmids purified by Qiagen really that bad?????
mkoch at usa.pipeline.com
Wed Apr 17 19:40:28 EST 1996
On Apr 17, 1996 04:50:55 in article <Are plasmids purified by Qiagen really
that bad?????>, 'The Wagner Lab <ewlab at uci.edu>' wrote:
We use the spin-column Quiprep kits (no resin etc). I use it for
sequencing directly out of the tube (no re-precipitation etc)
and only had good results. Works equally well for cloning.
>I've been talking to some people (more dislike it than like it, that's
>for sure) that really hate Qiagen plasmid preps because apparently the
>DNA often doesn't work as well as Cs purified for transfections and for
>in vitro transcription. Does anyone have any opinions (with experimental
>support, of course) to the contrary??
>I've also seen some sales literature for their endotoxin-free kits-- Does
>this mean that Qiagen is admitting a problem with purity??
>Just curious... Matt (Qiagen tech rep please don't contact me)
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