High G-C PCR...help.

Steven James Carlisle sjc4h at faraday.clas.Virginia.EDU
Wed Apr 17 11:39:50 EST 1996


I need advice on PCR...I've tried many times to amplify
a 1.2 Kb insert with multiple primers, different enzymes,
different templates, different TM's, and lots of bad
language...nothing.
It has a very high (about 75%) G-C content...is this a problem?
Anybody else had this experience?

steve
sjc4h at virginia.edu




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