Ligations with 1 cohesive & 1 blunt end...best conditions?

Kathy L kathryn at U.Arizona.EDU
Thu Apr 18 17:48:18 EST 1996


I am trying to do an insert/pCDNA3 ligation with T4 ligase in which both
pieces have one cohesive and one blunt end.  Was just wondering if anyone
knew what kind of conditions work best for this type of ligation.

Thanks for the help!

Kathy
kathryn at u.arizona.edu




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