Qiagen Maxi: Quantity
john.brennand at gbapr.zeneca.com
Thu Apr 18 04:43:13 EST 1996
Just a thought.
Is it possible that the problem is in the cultures - ie the bugs dont
contain as much plasmid as you think, because, for example, the
antibiotic levels are too low or it has gone off and plasmid free
segregants are overgrowing the culture ?
You could check this by 1st doing a miniprep from the culture, by an
alternative method, and then from the result predicting how much you
should get from the whole culture.
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