Qiagen Maxi: Quantity

john brennand john.brennand at gbapr.zeneca.com
Thu Apr 18 04:43:13 EST 1996


Just a thought.

Is it possible that the problem is in the cultures - ie the bugs dont 
contain as much plasmid as you think, because, for example, the 
antibiotic levels are too low or it has gone off and plasmid free 
segregants are overgrowing the culture ?

You could check this by 1st doing a miniprep from the culture, by an 
alternative method, and then from the result predicting how much you 
should get from the whole culture.


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