NdeI: cutting problems?? anyone else?? seems silly! (fwd)

Dr. Duncan Clark duncan at genesys.demon.co.uk
Thu Apr 18 02:42:19 EST 1996


In article <slrn4natef.7h.aiyar at ebv.oncology.wisc.edu>, Ashok Aiyar
<aiyar at ebv.oncology.wisc.edu> writes
>On 17 Apr 1996 21:56:24 GMT, gerry oneill <101733.2255 at CompuServe.COM> wrote:
>>Ndei cutting problems
>>
>>This may be due to the dam methylation status of the DNA sourse; ie 
>>dam +ve plasmid DNA
>>NdeI cannot restrict dan+ve DNA but its isochizomer Dpni can
>
>NdeI recognizes and cuts "CA/TATG".  There is neither a dam methylation
>site within that sequence, nor is NdeI an isoschizomer of DpnI,
>which recognizes and cuts "GAm/TC" (where Am is an N6 methylated dA).

I think he meant Nde II which is also present in the Nde I strain and is
an iso. of Mbo I. As Nde I from NEB is recombinant no Nde II will ever
be present in the purified enzyme prep.

Duncan 

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