Qiagen Maxi: Quantity

Gerd Romanowski Gerd.Romanowski at uni-bayreuth.de
Thu Apr 18 02:42:55 EST 1996


I have problems to get the promised quantity of DNA using the Qiagen Maxiprep. 
My max amount is 30 µg, not 500. I use DH10B with an OD of approx. 1. 100 ml 
of culture with pUC18 and 500 ml of Culture with pBR322. All my colleagues 
have the same problem, just one single case is known, where it has worked as 
promised :-) (hopeful). The quality of DNA is o.k.
The tech help of Qiagen just gave me the tips which are also in the manual.

Hope to hear some fine hints

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