Are plasmids purified by Qiagen really that bad?????
Joao Vasconcelos Costa
jcosta at pen.gulbenkian.pt
Thu Apr 18 02:53:45 EST 1996
Seng-Lai Tan wrote:
> We recently did a Qiagen prep of a library plasmid and used it for
> transformation of a yeast 2-hybrid strain (Hf7c). The transformation
> efficiency with this plasmid prep was fine in Hf7c. We were able to
> screen and identify interactive proteins with the library.
> However, for mammalian transfection, a much cleaner DNA prep is required.
My lab has a considerable experience in transfecting mammalian cells
with plasmid DNA. DNA is routinely prepared by Qiagen. We never had
***** JOAO VASCONCELOS COSTA, MD, PhD
***** Instituto Gulbenkian de Ciencia - Oeiras, Portugal
***** mailto:jcosta at pen.gulbenkian.pt
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