Are plasmids purified by Qiagen really that bad?????

Joao Vasconcelos Costa jcosta at pen.gulbenkian.pt
Thu Apr 18 02:53:45 EST 1996


Seng-Lai Tan wrote:
> 
> We recently did a Qiagen prep of a library plasmid and used it for
> transformation of a yeast 2-hybrid strain (Hf7c).  The transformation
> efficiency with this plasmid prep was fine in Hf7c.  We were able to
> screen and identify interactive proteins with the library.
> 
> However, for mammalian transfection, a much cleaner DNA prep is required.
> 
My lab has a considerable experience in transfecting mammalian cells
with plasmid DNA. DNA is routinely prepared by Qiagen. We never had
problems.

Joao

-- 
*****   JOAO VASCONCELOS COSTA, MD, PhD
*****   Instituto Gulbenkian de Ciencia - Oeiras, Portugal
*****   mailto:jcosta at pen.gulbenkian.pt
*****   http://www.pen.gulbenkian.pt/v2/jvc.html



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