HELP! 3-way ligation

Maria C. Costanzo mcc9 at
Thu Apr 18 16:33:51 EST 1996

We're having trouble doing a 3-way ligation in which the vector has PstI
and BamHI ends, one insert piece has BamHI and NcoI ends, and the other
insert piece has BspHI (compatible with NcoI) and PstI ends.  Does anyone
have any tips on strategies for 3-way ligations in general or any info
about these particular sticky ends?
Thanks in advance,

Maria C. Costanzo
Section of Genetics & Development
Biotechnology Building
Cornell University
Ithaca, NY 14853-2703
(607) 254-4834
mcc9 at

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