How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers?

[Jose M. Bautista]

On 18 Apr 1996, Ruud Ross wrote:

> Dear Netters,
> 
> I'm just curious. How many bases can be read with ALF-sequencers, from
> Pharmacia. Who has good results 
> and what kind of conditions do you use? Are ALF-sequencers better then other,
> like ABI-sequencers?
> Thanks in advance.
> 
> Ruud Ross
> r.ross at organon.akzonobel.nl
> 
> 


We have been sequencing about 50 kbp/year in my lab, most of them with 
the ALF, and now we have changed to Perkin. The worst are the ALF-sequencers 
from Pharmacia for a lot of different reasons. 
Go for a Perkin-Elmer. No comparison at all.

.................................................................
.       Jose M. Bautista                                        .
.       Department of Haematology                               .
.       Royal Postgraduate Medical School                       .
.       Hammersmith Hospital, Du Cane Road, London W12 0NN,UK   .
.       Tel + 44 181 743 2030 ext. 2162                         .
.       Fax + 44 181 742 9335                                   .
.       e-mail boxes:   bauchem at eucmax.sim.ucm.es               .
.                       jbautist at hgmp.mrc.ac.uk                 .
.                       jmbautista at samba.cnb.uam.es             .
.................................................................