minfa at PANTHER.CMB.UMU.SE
Thu Apr 18 11:25:13 EST 1996
I have a protein with only two cysteines. I would like to label
these cysteins with spin-label probe to measure the distance
between these two residues. I got the probe called 4-(2-Iodoacetamide)-TEMPO
My questions are:
1. How to detect the efficiency of incorparation of the probe to protein.
2. Do I need 100% labeling (both -SH groups in cysteines are
coupled to spin probe in one molecule). Will single-labeled
protein (only one cysteine is on) effect EPR spectrum to
deviate the distance measurement.
Looking forward to getting the advice from experts. Thank you in
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