Peptide transfer to PDVF

Ming Fa minfa at panther.cmb.umu.se
Fri Apr 19 03:01:22 EST 1996


Hey everybody,

I have a mixture of peptide fragments from 2 kDa to 20 kDa.  I can see all these peptides on polyacrylamide gel, but after transfer to PDVF membrane (Western blotting according to Towbin) I can detect only fragments bigger than 9 kDa. Is it possible that peptides smaller than 9 kDa do not bind to PDVF membrane?  If yes, does anyone know how to improve the transfer of peptide?
                                    
                                    Thanks in advance,
                                    
                                    Malgorzata Wilczynska
                                    Medical Biochemistry
                                    Umeå University
                                    margaret at solaris.chem.umu.se.




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