Peptide transfer to PDVF
minfa at panther.cmb.umu.se
Fri Apr 19 03:01:22 EST 1996
I have a mixture of peptide fragments from 2 kDa to 20 kDa. I can see all these peptides on polyacrylamide gel, but after transfer to PDVF membrane (Western blotting according to Towbin) I can detect only fragments bigger than 9 kDa. Is it possible that peptides smaller than 9 kDa do not bind to PDVF membrane? If yes, does anyone know how to improve the transfer of peptide?
Thanks in advance,
margaret at solaris.chem.umu.se.
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