Trizol RNA purity
kathryn at U.Arizona.EDU
Fri Apr 19 14:34:20 EST 1996
On 19 Apr 1996, Edward Wang wrote:
> I am currently using Trizol from gibco for my total RNA prep. Out of
> curiosity, what are the 260/280 reading people are gettin? Also, has
> anyone used the reagents with tissues with high RNAse...And did you have
> to take extra precautions to prevent the RNA from getting chewed up?
Well, I've been using Trizol for awhile and my 260/280 readings always
seem to be about 2.0-2.1. Been using it on RNA from mammalian cells
though, not tissues, but that shouldn't make any difference.
kathryn at u.arizona.edu
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