Precipitation of DNA/RNA with isopropanol

Bill Alexander alexanderw at cber.cber.fda.gov
Fri Apr 19 12:36:05 EST 1996


In Article <4l8d54$7oj at news.duke.edu>, Margon Vandongen
<vando006 at mc.duke.edu> wrote:
>Eric,
>
>Are you assuming there is already salt in the DNA preparations??
>Otherwise you will have to add salt to precipitate DNA or RNA no matter 
>if you use ethanol or isopropanol.The only difference is that you can add
>less volume with isopropanol than of ethanol (equal volume versus 2-3
>times volume of final sample.)
>
>Margon
>
The salt you should use may also varry depending what you want to do with
your nucleic acids.

See:  Molecular Cloning, A Laboaratory Manual; Second Ed.  Sambrook et al.  
Book 3, pages E.10-E.11
Regards,

Bill Alexander
alexanderw at cber.cber.fda.gov

     "640K ought to be enough for anybody." -- Bill Gates, 1981



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