Help with high mol wt protein stds
vidhya at osuunx.ucc.okstate.edu
Sun Apr 21 19:57:51 EST 1996
I have couple of proteins that are more than 200 kDa on SDS-page.
I need to find the exact mol. size of these. I ahve tried
sigma's phosphorylase b oligomer stds but my proteins don't
get resolve at the acrylamide conc they suggest in the
tech bulletin. Does anybody have any experience/suggestions?
I have no means of assaying the activity of the protein so
can't do any gel filtration to monitor it.
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