Help with high mol wt protein stds

Vidhya Rangaswamy vidhya at osuunx.ucc.okstate.edu
Sun Apr 21 19:57:51 EST 1996


I have couple of proteins that are more than 200 kDa on SDS-page.
I need to find the exact mol. size of these.  I ahve tried
sigma's phosphorylase b oligomer stds but my proteins don't
get resolve at the acrylamide  conc they suggest in the 
tech  bulletin.  Does anybody have any experience/suggestions?

I have no means of assaying the activity of the protein so
can't do any gel filtration to monitor it.

Thanks
Vidhya





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