[solubilization of inclusion bodies with SDS?]

Axel Schumacher as at macpost.pharmakologie.uni-erlangen.de
Sun Apr 21 03:20:43 EST 1996


In article <4kvb0b$sqr at nuscc.nus.sg>, scip3090 at leonis.nus.sg (Tan Nguan
Soon) wrote:

> Can anyone please help me? I have a question, i.e., can proteins in 
> inclusion bodies be solubilize using 1x sample buffer (containing SDS, 
> b-mercap.) followed by 5 min of boiling?
> 
> thank you.

Yes, for subsequent gel elctrophoresis that works fine.

Axel

-- 
Axel Schumacher, Ph.D.     ! Phone:+49 9131 852002
Institute for Pharmacology ! FAX:  +49 9131 206119
University of Erlangen     !
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D-91054 Erlangen  Germany  ! as at macpost.pharmakologie.uni-erlangen.de




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