blunt&sticky-1 Ligation?

Mark Henteleff markh at ahi.pbrc.hawaii.edu
Mon Apr 22 16:01:37 EST 1996


In article <Dq9wpA.85G at ncifcrf.gov>,
Paul N Hengen <pnh at cockleberry.ncifcrf.gov> wrote:
>Georg Kroeger (a03m at biologie.uni-bremen.de) wrote:
>
>| I have been trying to ligate a small piece of dsDNA (60bps,
>| constructed by building overlapping sense and antisense oligos
>| with restriction site overhangs at each end) into a cDNA with
>| corresponding restriction sites.  The sites are Nsi (4bp overhang)
>
>-> löasdflasdf nwdifasd lf weoifadf fd
>
>Obviously, this is the anti-sense message ;-)
>
>-Paul.

Gee guys, thanks for all the help.
Mark H. (the guy with the ligation problems)




More information about the Methods mailing list