blunt&sticky-1 Ligation?

Mark Henteleff markh at
Mon Apr 22 16:01:37 EST 1996

In article <Dq9wpA.85G at>,
Paul N Hengen <pnh at> wrote:
>Georg Kroeger (a03m at wrote:
>| I have been trying to ligate a small piece of dsDNA (60bps,
>| constructed by building overlapping sense and antisense oligos
>| with restriction site overhangs at each end) into a cDNA with
>| corresponding restriction sites.  The sites are Nsi (4bp overhang)
>-> löasdflasdf nwdifasd lf weoifadf fd
>Obviously, this is the anti-sense message ;-)

Gee guys, thanks for all the help.
Mark H. (the guy with the ligation problems)

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