hybing without shaking??
lelyc at u.washington.edu
Mon Apr 22 19:50:41 EST 1996
I was doing an overnight hybe of some Northern and RNA dot blots in a
shaking waterbath. Something went wrong and the waterbath had stopped
shaking when I came in the next morning. Is shaking absolutely necessary
for a good signal?? I had 4 blots in 50 mls of hybe solution in a small
pyrex container. There was just enough hybe to cover all of the blots and
still allow for diffusion between blots.
Thanks in advance,
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