hybing without shaking??

Lely Chow lelyc at u.washington.edu
Mon Apr 22 19:50:41 EST 1996


Hi,

I was doing an overnight hybe of some Northern and RNA dot blots in a
shaking waterbath.  Something went wrong and the waterbath had stopped
shaking when I came in the next morning.  Is shaking absolutely necessary
for a good signal??  I had 4 blots in 50 mls of hybe solution in a small
pyrex container.  There was just enough hybe to cover all of the blots and
still allow for diffusion between blots.

Thanks in advance,

--Lely







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