Not sizing cDNA (random primed)?

James Graham graham at
Mon Apr 22 19:13:09 EST 1996

I've run across the suggestion that without any size selection 
of random primed cDNA, there might be problems in adapter ligation
and sucloning to cDNA libraries. I am only interested in getting
the maximum amount of sequence into libraries rather than 
defining trascription units.

Any known problems with cloning unsized total cDNA from a random
primed synthesis?

E-mail copies please,

J. Graham PhD 
Biology Department 
Washington University of St. Louis 

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