Not sizing cDNA (random primed)?

James Graham graham at biodec.wustl.edu
Mon Apr 22 19:13:09 EST 1996


I've run across the suggestion that without any size selection 
of random primed cDNA, there might be problems in adapter ligation
and sucloning to cDNA libraries. I am only interested in getting
the maximum amount of sequence into libraries rather than 
defining trascription units.

Any known problems with cloning unsized total cDNA from a random
primed synthesis?

E-mail copies please,

Jim
J. Graham PhD 
Biology Department 
Washington University of St. Louis 



More information about the Methods mailing list