Not sizing cDNA (random primed)?
graham at biodec.wustl.edu
Mon Apr 22 19:13:09 EST 1996
I've run across the suggestion that without any size selection
of random primed cDNA, there might be problems in adapter ligation
and sucloning to cDNA libraries. I am only interested in getting
the maximum amount of sequence into libraries rather than
defining trascription units.
Any known problems with cloning unsized total cDNA from a random
E-mail copies please,
J. Graham PhD
Washington University of St. Louis
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