Vector as cDNA primer??

James Graham graham at biodec.wustl.edu
Mon Apr 22 18:46:52 EST 1996


I have noticed Rubenstein's NAR 18 (16) p.4833 1990 in which he 
describes a method for making cDNA libraries using a "tailed" 
linearized vector as the "primer" for cDNA synthesis.

This presumably could lead to a much improved efficiency of sub-cloning
following synthesis, which would seem to be a major factor 
in working with limiting amounts of mRNA (100 ng cDNA?).

Anyone try this or find other important angles on this problem?
(Overall goal is subtractive hybridization of cDNA libraries).

Please E-mail copies,

Jim
J. Graham PhD 
Biology Department 
Washington University of St. Louis 

 



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