hybing without shaking??

[Pam Snyder]

In article
<Pine.A32.3.92a.960422174617.112714A-100000 at homer05.u.washington.edu>,
Lely Chow <lelyc at u.washington.edu> wrote:

> Hi,
> 
> I was doing an overnight hybe of some Northern and RNA dot blots in a
> shaking waterbath.  Something went wrong and the waterbath had stopped
> shaking when I came in the next morning.  Is shaking absolutely necessary
> for a good signal??  I had 4 blots in 50 mls of hybe solution in a small
> pyrex container.  There was just enough hybe to cover all of the blots and
> still allow for diffusion between blots.
> 
> Thanks in advance,
> 
> --Lely

We typically hyb without shaking, as long there was enough hyb they should
be fine.  ( aside :When you are hybridizing that many filters at one time
have you every thought of using a fine mesh in between?  it can reduce the
volume of hyb needed, and prevent blank spots)