blunt&sticky-1 Ligation?

Georg Kroeger a03m at
Mon Apr 22 14:36:55 EST 1996

markh at (Mark Henteleff) wrote:

>I have been trying to ligate a small piece of dsDNA (60bps,
>constructed by building overlapping sense and antisense oligos
>with restriction site overhangs at each end) into a cDNA with
>corresponding restriction sites.  The sites are Nsi (4bp overhang)

löasdflasdf nwdifasd lf weoifadf fd

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