Asymmetric PCR

Doug Johnson djohnson at oreo.uottawa.ca
Mon Apr 22 16:10:05 EST 1996


I would appreciate hearing your opinions of the use of asymmetric PCR
for the preparation of single-stranded DNAs. The DNAs would be used
as probes of RNA blots with the intention of identifying the coding
and non-coding strands. (I am considering the PCR approach instead of
althernatives such as RNA probes or oligomers).

Thanks for your help and all references appreciated. 

Doug Johnson,
djohnson at oreo.uottawa.ca



More information about the Methods mailing list