Asymmetric PCR

Doug Johnson djohnson at
Mon Apr 22 16:10:05 EST 1996

I would appreciate hearing your opinions of the use of asymmetric PCR
for the preparation of single-stranded DNAs. The DNAs would be used
as probes of RNA blots with the intention of identifying the coding
and non-coding strands. (I am considering the PCR approach instead of
althernatives such as RNA probes or oligomers).

Thanks for your help and all references appreciated. 

Doug Johnson,
djohnson at

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