Plasmids purified by Qiagen - just fine!
nikolaic at VISAR.WUSTL.EDU
Mon Apr 22 14:55:07 EST 1996
> Date: Thu, 18 Apr 1996 10:03:43 -0700
> In-Reply-To: <31759E90.16F2 at visar.wustl.edu> Seng-Lai Tan wrote:
> I guess it also depends on what mammalian cells you're transfecting.
> Some are really picky and need very clean DNA. Do your Qiagen preps work
> for all your cells? Just curious.
> On 17 Apr 1996, Nikolai Chitaev wrote:
> > Dear Seng-Lai Tan,
> > I`m not affiliated with Qiagen by any means, with exeption that our lab routinly
> > use plasmid dna purified with Qiagen mini-preps for all possible cloning work, and
> > Qiagen maxi-preps for stable transfection of different mammalian cells.
> > Everything works just fine. During three years of work -, no one single problem.
> > I`m writing it not to say the Qiagen`s dna the best, but to say: before you blame
> > the kit be sure you did everything right. Good luck.
> > --
> > Nikolai
> > http://184.108.40.206
So far I`ve got stable transfected clones from HaCaT, HT1080, p2a, A431 human cells.
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