RNA Molecular Weight Marker

Patrick Hurban hurban at cmgm.stanford.edu
Mon Apr 22 10:16:28 EST 1996


In article <4lg1rs$n2i at elna.ethz.ch>, Markus Ruf <ruf at ilw.agrl.ethz.ch> wrote:

> Hi everybody,
> 
> Have got problems with my Northern blots. Actually not the blot is the
> problem, but my RNA molecular weight marker. I used the marker from 
> Boehringer and have done the staining as recommended in EtBr, but I can't 
> get anything visible (Boehringer writes: Bands may not be visible to the 
> naked eye, although they show up clearly on photograph !!!). 
> 
> Has got somebody an idea, how I can get the bands visible?
> 
> 
> Thanks in advance
> 
>         Tom
> 
> 
> **********************
> Tom Geissmann        *
> Food Mikrobiology    *
> ETH Zurich           *
> ruf at ilw.agrl.ethz.ch *
> **********************

Try using the RNA markers from GIBCO BRL.  We routinely use 20 ng/lane. 
Instead of staining for them, we hybridize with a probe made from BRL's 1
kb DNA ladder (about 50,000 cpm/ml hybridization fluid).  Works like a
charm.

-- 
Patrick Hurban



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