Silvia Spitzer SSPITZER at path.som.sunysb.edu
Mon Apr 22 08:53:27 EST 1996

In article <3178073A.41C67EA6 at tuba.calpoly.edu>, Anonymous
<smccullo at tuba.calpoly.edu> wrote:

> Hello,
> I can't seem to find the answer to this in any of the books I have lying
> around, so please forgive me if this is an inane question.
> What exactly does CTAB do? I'm using it when doing DNA extractions from
> environmental samples(papaer originally by Boom, et al.) I remember
> being told from a semi-reliable source it was a protein denaturant, but
> SIgma lists it as useful in DNA precipitation.  Thanks In advance,
It removes polysacharides that will otherwise coprecipitate with the DNA,
yielding an "unusable DNA" (undigestable, unligatable, even unprecipitable
if polysacharide concentration is very high).
SSPITZER at path.som.sunysb.edu

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