CTAB....

Silvia Spitzer SSPITZER at path.som.sunysb.edu
Mon Apr 22 08:53:27 EST 1996


In article <3178073A.41C67EA6 at tuba.calpoly.edu>, Anonymous
<smccullo at tuba.calpoly.edu> wrote:

> Hello,
> 
> I can't seem to find the answer to this in any of the books I have lying
> around, so please forgive me if this is an inane question.
> 
> What exactly does CTAB do? I'm using it when doing DNA extractions from
> environmental samples(papaer originally by Boom, et al.) I remember
> being told from a semi-reliable source it was a protein denaturant, but
> SIgma lists it as useful in DNA precipitation.  Thanks In advance,
> 
>
It removes polysacharides that will otherwise coprecipitate with the DNA,
yielding an "unusable DNA" (undigestable, unligatable, even unprecipitable
if polysacharide concentration is very high).
Silvia
SSPITZER at path.som.sunysb.edu



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