hybing without shaking??

Ned Mantei mantei at neuro.biol.ethz.ch
Tue Apr 23 00:32:59 EST 1996

In article
<Pine.A32.3.92a.960422174617.112714A-100000 at homer05.u.washington.edu>,
Lely Chow <lelyc at u.washington.edu> wrote:

>Is shaking absolutely necessary for a good signal??

Shaking is definitely not necessary. If you use hybridization buffer with
50% formamide, it suffices to simply moisten each filter (for colony
lifts, for example,1--2 ml buffer per filter; to be sure that all filters
are evenly wet, put one filter at a time into a Petri dish and cover with
aliquot of buffer before adding the next filter.)

Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046

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