hybing without shaking??
colin at fungus.biochem.ualberta.ca
Wed Apr 24 00:38:16 EST 1996
Lely Chow wrote:
> I was doing an overnight hybe of some Northern and RNA dot blots in a
> shaking waterbath. Something went wrong and the waterbath had stopped
> shaking when I came in the next morning. Is shaking absolutely necessary
> for a good signal?? I had 4 blots in 50 mls of hybe solution in a small
> pyrex container. There was just enough hybe to cover all of the blots and
> still allow for diffusion between blots.
Not shaking won't likely hurt anything.
> Thanks in advance,
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