hybing without shaking??

[Colin Rasmussen]

Lely Chow wrote:
> 
> Hi,
> 
> I was doing an overnight hybe of some Northern and RNA dot blots in a
> shaking waterbath.  Something went wrong and the waterbath had stopped
> shaking when I came in the next morning.  Is shaking absolutely necessary
> for a good signal??  I had 4 blots in 50 mls of hybe solution in a small
> pyrex container.  There was just enough hybe to cover all of the blots and
> still allow for diffusion between blots.

Not shaking won't likely hurt anything.

Colin
> 
> Thanks in advance,
> 
> --Lely