[solubilization of inclusion bodies with SDS?]
mikef at mjr.com
Tue Apr 23 17:24:42 EST 1996
In article <as-2104961021160001 at klinpharm.pharmakologie.uni-erlangen.de>,
as at macpost.pharmakologie.uni-erlangen.de (Axel Schumacher) wrote:
>In article <4kvb0b$sqr at nuscc.nus.sg>, scip3090 at leonis.nus.sg (Tan Nguan
>> Can anyone please help me? I have a question, i.e., can proteins in
>> inclusion bodies be solubilize using 1x sample buffer (containing SDS,
>> b-mercap.) followed by 5 min of boiling?
>Yes, for subsequent gel elctrophoresis that works fine.
And in some cases, you can do even more. I found that at least one protein
could be refolded into a functional DNA-binding protein, even after
boiling, just by dialysing away the SDS.
Don't count on this working in most cases.
Michael Finney, PhD | /\/\\_\ /\_\ | 149 Grove Street
MJ Research, Inc. | /\_\_\\ \\_\ | Watertown MA 02172
mikef at mjr.com | /_/ | 617 923 8001 x112
More information about the Methods