help with semiQ RT-PCR

Robert K Furse PhD rfurse
Tue Apr 23 08:52:41 EST 1996


Without further knowledge of your system I am left to wonder about your primer
concentration.  If you have an xs of primers you should be able to see
proportional increases in your mutant product as you add more mutant DNA to the
reaction.  I'll continue to think about this one




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