Vector as cDNA primer??

john brennand john.brennand at
Tue Apr 23 04:10:33 EST 1996


This is old technology first published around 1982 by Okayama & Berg, 
Pharmacia used to sell the bits to do it.

We played around a bit with it in the early days with limited success - 
it worked but our library sizes were small - we didn't persevere with 

However with all the improvements in enzyme quality, seperation 
matrices, transformation frequencies etc, this must be an attractive 


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