Vector as cDNA primer??
john.brennand at gbapr.zeneca.com
Tue Apr 23 04:10:33 EST 1996
This is old technology first published around 1982 by Okayama & Berg,
Pharmacia used to sell the bits to do it.
We played around a bit with it in the early days with limited success -
it worked but our library sizes were small - we didn't persevere with
However with all the improvements in enzyme quality, seperation
matrices, transformation frequencies etc, this must be an attractive
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