help with semiQ RT-PCR
Janet E. Rubin
jrubi02 at larry.cc.emory.edu
Wed Apr 24 08:43:53 EST 1996
Bernard Murray (bernard at elsie.nci.nih.gov) wrote:
: As I say I have strong objections to most of this but if the journal
: wants to accept it then I can't see people complaining. When the
: manuscript is published I'll pass on the actual reference.
: Strange days indeed......
Unfortunately that hasn't been my experience, with grants or papers. It
seems to be a very political thing, or perhaps dependent only on the
success of the reviewer to get his/her technique going. I guess I think
it's kind of silly - in the end mRNA is only a signal - if you want to
know what's there you have to have the protein. But I do believe that
RT-PCR gives you an idea about *relative* changes in steady state mRNA in
the expression systems.
Janet Rubin, MD
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