In Situ HELP

Chris Regan regan at phy164.physiology.uab.edu
Wed Apr 24 09:41:02 EST 1996


Hello.
I was wondering if anyone had a good protocol for in situ 
hybridization?  I have formalin fixed, paraffin embedded tissue and 
would like to use a chromagen labelled probe
Please also indicate how "clean" you need to be in the tissue 
collecting, secitioning, and incubation steps.  I have heard two 
opinions on this, some say RNAse free while others aren't that 
concerned.
Thanks in advance.
-- 
Chris Regan																										
Regan at phy164.physiology.uab.edu
http://phy184.physiology.uab.edu																																		
Department of Physiology																
Univ. of Alabama - Birmingham



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