Band-shift assay with U937

Ralph Melcher melcher at
Wed Apr 24 14:05:24 EST 1996

Hi netters,
I want to make a band-shift assay with nuclear cell extract from 
U937-cells with a 370 bp DNA-promoter-fragment. I am searching for a
regulatory region responsible for steroid hormon stimulation ( Calcitriol ).
Does anyone has experiences with band-shifts and footprinting, especially with 
U937 cells.

Many thanks

Beatrice Bodammer
Institut für Physiologische Chemie

email:Bodammer at 

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