Quantitative RT-PCR on blood

P. Mertens mertens at toxi.ucl.ac.be
Wed Apr 24 07:22:04 EST 1996


Among all available methods or kits to obtain RNA (either total or mRNA), 
 what would be the best to obtain starting material for quantitative 
RT-PCR from blood? The available blood quantity will be less than half a 
milliliter.
I'm currently using TRIzol with success to purify RNA from tissue 
samples. Does anyone have comments about the TRIzol-LS for liquid 
samples?
Any comments will be appreciated.
Pascal




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