blunt&sticky-1 Ligation?

Ferland Louis H. ferlandl at ERE.UMontreal.CA
Thu Apr 25 00:01:28 EST 1996


On Mon, 22 Apr 1996, Paul N Hengen wrote:

> Date: Mon, 22 Apr 1996 17:03:10 GMT
> From: Paul N Hengen <pnh at cockleberry.ncifcrf.gov>
> To: methods at net.bio.net
> Subject: Re: blunt&sticky-1 Ligation?
>=20
> Georg Kroeger (a03m at biologie.uni-bremen.de) wrote:
>=20
> | I have been trying to ligate a small piece of dsDNA (60bps,
> | constructed by building overlapping sense and antisense oligos
> | with restriction site overhangs at each end) into a cDNA with
> | corresponding restriction sites.  The sites are Nsi (4bp overhang)
>=20
> -> l=F6asdflasdf nwdifasd lf weoifadf fd
>=20
> Obviously, this is the anti-sense message ;-)
>                        ^^^^^^^^^^
                         ||||||||||
> -Paul.
>=20

Yo, Paul!
I thought we had BANNED this word for dsDNA!! ;-)}

-Louis

Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757     FAX: (514) 843-2719




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