High G-C PCR...help.

Rae Nishi nishir at ohsu.edu
Wed Apr 24 17:39:04 EST 1996


We banged our heads against the wall for quite awhile trying to amplify
a sequence that is 74% G + C.  It would only work with vent (exo- gave
more product than intact vent) at a melting temp of 96 degrees C.  Taq
was useless.  

Rae Nishi, PhD
Associate Professor
Dept. Cell & Developmental Biology
Oregon Health Sciences University
Portland Oregon 97201
**that's Orygun, NOT Ora-Gone**



More information about the Methods mailing list