ability to pcr introns from cDNA

Nancy Duncan duncanna at ohsu.edu
Fri Apr 26 18:28:48 EST 1996


I was wondering if anyone has an opinion on the ability to pcr introns 
from a cDNA prep...I understand that cDNA by definition is exon only, but 
is it possible to isolate some pre-mRNA, and then reverse transcribe that 
to cDNA, which could lead to pcr amplification of introns? We hope the 
answer is no, but are unsure....thanks....




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