Subtractive Hybridization Pitfalls?

[Pete Muriana]

In article <4lp3gu$5ik at mozo.cc.purdue.edu
> cwen at aux.btny.purdue.edu (Chi-Kuang Wen) writes:
>Dear Peter:
>        When working on the prokaryotic genomic DNA subtraction, the 
>complexity of the DNA is a lot smaller compared with the eukaryotic genomic 
>DNA or cDNA.  In your work, the genomic DNA is only 3Mbp, considering if  the 

..........pontification on differences between working with eukaryotic DNA vs. 
          prokaryotic DNA deleted :-)

>  A lot more >difficulties will be encountered when working with 
organisms with complex DNA >composition.     
>        Regards,
>Chi-Kuang

Dear Chi-Kuang,  the original poster did not indicate the need for information 
on eukaryotic or prokaryotic DNA.  I recognized that fact and qualified my 
statements indicating the procedures I have used worked well with total
"prokaryotic" DNA since these microorganisms don't have the luxuries of 
eukaryotic organisms of being able to "pull out" poly-A tailled mRNA.  
Actually, the original poster requested "any" comments regarding "subtractive 
hybridization" to which I responded.  If you feel the need to "lecture" 
someone, please give one in your facility.
Regards, 
Peter Muriana





















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>Chi-Kuang Wen
>Botany & Plant Pathology
>Purdue University
>West Lafayette, IN 47907
>USA

>fax(work): (317)494-5896
>phone(work):(317)494-0661

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   Peter M. Muriana 
   Assistant Professor                         317-494-8284   TEL            
   Dept. of Food Science                     317-494-7953   FAX            
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