Dichloromethane vs. Chloroform

acatalan at EXTRO.UCC.SU.OZ.AU acatalan at EXTRO.UCC.SU.OZ.AU
Mon Apr 29 03:01:12 EST 1996


A past posting following a query about using dichloromethane instead of 
chloroform for DNA extractions suggested that there was no safety advantage.

I looked up the msds for both chemicals and found that chloroform is by far
more hazardous than dichloromethane.

If dichloromethane does work as well as chloroform then perhaps the
molecular biology community should start replacing chlorofom in their
protocols (for their own health's sake)!

The molecular biology community is often very conservative when it
comes down to "standard" protocols.

What do the bionetters think?
Let's re-open this issue!

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|  Alberto Catalano                                                   |
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>
>                          RE: CHLOROFORM REPLACEMENT?
>                                       
>   s060965 at aix1.uottawa.ca
>   7 Sep 1995 08:19:41 -0700
>   
>
>To: methods-and-reagents at net.bio.net
>
>From: s060965 at aix1.uottawa.ca
>
>Subject: Re: Chloroform Replacement?
>
>Date: 7 Sep 1995 08:19:41 -0700
>
>   
>   
>   
>   
>   Dichloromethane is not much less toxic than chloroform.
>   
>   
>   On 7 Sep 1995, David R. Rockhold wrote:
>   
>   
>   > Considering the toxicity of chloroform...has anyone tried
>   dichloromethane as
>   > a substitute for chloroform in nucleic acid purification schemes?
>   > Dave Rockhold, Microbiologist Phone 510-559-5722
>   > 800 Buchanan Street Fax 510-559-5775
>   > Albany, CA 94710 
>   > 
>   > 
>   
>     _________________________________________________________________
>   




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