in situ pcr protocol for bacteria?

Ferenc Marincs agpfxm at pnv.palm.cri.nz
Tue Apr 30 00:37:55 EST 1996


In article <4lqh2g$p4i at studium.student.umu.se>, david.haydon at micro.umu.se
(David Haydon) wrote:

> We need a protocol for doing in-situ PCR as a screen for mutants on 
> colonies of E.coli.
> 
> Any advice/protocols/references would be greatly appreciated.
> 
> Many thanks in advance
> 
> David
> -- 
>                          \\|//
>                          (o o)
>  / }------------------oOO-(_)-OOo----------------------/ }
> { /                                                   { /
>  X                  Dr David Haydon                    X
> { \             David.Haydon at micro.umu.se             { \
>  \ }                                                   \ }
>   X              "To err is to be human,                X
>  / }    __o    but to mess things up completely        / }
> { /  _-\_<,       requires a computer!"               { /
>  X  (*)/'(*)                                           X
> { \---------------------------------------------------{ \



The reference is: Hodson et al. in Appl. Environ. Microbiol. Vol.61 pp.:
4072-4082.

Frank

Ferenc Marincs
AgResearch, Private Bag 11008, Palmerston North, New Zealand
Phone: +64-6-356 8019, Fax: +64-6-351 8032
E-mail: agpfxm at pnv.palm.cri.nz



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