Blood spot DNA Extraction

John Christodoulou johnch at postbox.su.edu.au
Tue Apr 30 00:13:44 EST 1996


I am interested in being able to extract DNA from dried blood spots 
(Guthrie cards) for PCR and allele specific oligonucleotide analysis 
using the Amersham ECL kit.  We have found that using simple 
extraction methods and amplification we get nonspecific binding of the 
labelled probe, which we suspect is due to something else coeluting 
from the Guthrie card.

     Has anyone used the Amersham kit with amplified DNA from blood 
spots??  Cheers.



John Christodoulou
Dept of Paediatrics & Child Health
University of Sydney
Sydney



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